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Grade 9 · Lesson 3 of 12 · about 12 min

Asexual Reproduction: Fission, Budding, Spores, Cloning and Grafting

How one parent makes genetically identical offspring: the five forms of asexual reproduction in the BC curriculum, with advantages and disadvantages.

🎯 By the end of this lesson

  • Students should be able to define asexual reproduction and explain why the offspring are genetically identical to the parent.
  • Students should be able to describe the steps of binary fission and calculate population growth by repeated doubling.
  • Students should be able to describe budding and give two examples.
  • Students should be able to distinguish asexual spores from sexual spores.
  • Students should be able to describe natural and artificial cloning, including the steps of reproductive cloning.
  • Students should be able to define scion and stock and explain how grafting works.
  • Students should be able to list advantages and disadvantages of asexual reproduction.
  • Students should be able to plan a fair test about an asexual reproduction question.

1Reproducing without a partner

A single bacterium dropped into a nourishing broth can, in a matter of hours, become a cloudy swarm of millions of bacteria, all descended from that one cell. No second parent, no mating, no courtship. How is that possible?

The answer is asexual reproduction: reproduction in which a single parent produces offspring that are genetically identical to itself. The offspring are clones of the parent. Asexual reproduction is built on the cell division covered in the previous two lessons. Because the offspring come from copies of the parent's own DNA, with no mixing of genes from a second individual, they carry the same genetic instructions as the parent.

Key idea

Asexual reproduction needs only one parent and produces genetically identical offspring (clones). The BC curriculum lists five forms to know: fission, budding, cloning, spores and grafting.

Asexual reproduction is not rare. It is how bacteria multiply, how yeast reproduces, how many fungi spread, and how gardeners multiply favourite plants. This lesson looks at each of the five forms, then compares the strengths and weaknesses of reproducing this way.

2Fission: splitting in two

Fission means the parent organism divides into two individuals of roughly equal size. The classic example is the binary fission of bacteria.

Binary fission step by step

Bacteria are prokaryotes. They have no nucleus and usually a single circular chromosome, so they do not use mitosis. Their method is simpler.

  1. Growth. The cell gets larger and builds up its internal components.
  2. DNA replication. Copying begins at a specific starting point on the circular chromosome, which is attached to the inner membrane, and proceeds in both directions until the whole chromosome has been copied. The two copies move apart.
  3. Septum formation. A protein called FtsZ assembles into a ring at the middle of the cell. Other proteins join it and build new cell-wall material inward across the middle, forming a dividing wall called a septum.
  4. Division. The septum grows inward and the cell constricts until it splits into two daughter cells. Each has a full copy of the chromosome and a share of the cytoplasm.
Binary fission: one cell becomes two1. One circularchromosome2. DNA is copied;copies move apart3. Cell lengthens;a wall forms (septum)4. Twoidentical cellsstart: 11 division: 22 divisions: 43 divisions: 8Doubling each time gives exponential growth.
Binary fission (top) and the doubling of cell numbers (bottom).

Why fission leads to such rapid growth

The time a population takes to double through one round of fission is its generation time (doubling time). Because every division doubles the number of cells, growth is exponential. The number of cells after n generations is the starting number multiplied by 2n. The doubling time of E. coli can be as short as about 20 minutes under ideal laboratory conditions, though some bacteria need days in harsh environments.

Worked example

A culture begins with 1 × 105 cells (100 000 cells), and the cells divide every 30 minutes. How many cells are there after 2 hours, assuming none die?

Two hours is 4 half-hour periods, so there are 4 doublings. The population is 100 000 × 24 = 100 000 × 16 = 1 600 000 cells (1.6 × 106).

Following the same pattern for a day, one cell dividing every 30 minutes would complete 48 divisions in 24 hours, giving 248, or about 2.8 × 1014, cells in theory. In reality, food runs out and wastes build up long before that, which stops the growth.

Fission is not limited to bacteria. Some single-celled organisms with a nucleus divide in two by mitosis. A few multicellular animals also reproduce by splitting, including sea anemones, coral polyps and some sea stars (which split the central disk of the body).

3Budding: a small copy grows from the parent

In budding, a small outgrowth forms on the body of the parent and develops into a new individual. Unlike fission, the parent and offspring are initially very different in size.

  • Yeast. A bud forms on the surface of a yeast cell and shares cytoplasm with the parent. The parent's nucleus divides, and one copy moves into the bud. A parent can produce several buds, and buds sometimes form chains before they separate.
  • Hydra. This small animal grows a bud on its body. The bud develops into a miniature adult and breaks away.
  • Corals. The bud stays attached and becomes part of a growing colony.
Budding and sporesBudding in yeasta small bud grows on theparent, then separatesBudding in hydraa bud develops into asmall copy and detachesSpores from a fungustiny cells scatter and cangrow into new fungiNo fertilization is needed in any of these cases.
Budding in yeast and hydra, and spore release from a fungus.
Common misconception

“Budding is just a plant thing, as in flower buds.” The word has two meanings. A flower or leaf bud is a young shoot of a plant. In reproduction, budding means that a new individual grows out of the body of the parent. Yeast and hydra are the standard examples.

4Spores: tiny packages for spreading

A spore is a tiny reproductive cell that can grow into a new individual without fertilization. Fungi are the best-known spore producers. After a period of growth, a fungus releases very large numbers of spores. Spores are usually single cells, made either by breaking pieces off the fungal threads (the mycelium) or inside specialized structures such as sporangia.

FeatureAsexual sporesSexual spores
Made byOne parent, no partner (cell division by mitosis)Fusion of two gametes, bringing two nuclei together
OffspringGenetic copy of the parentGenetically different from either parent
Also calledMitospores(see the variation lesson)

Only asexual spores count as asexual reproduction. A few other fungal methods are also asexual: the fungal body can break into fragments that each grow into a new fungus, and some single-celled yeasts simply divide into two. Buds that break off from a thread-like fungal body (hypha) can act as spores.

5Cloning: making genetically identical copies

Cloning refers to methods for making genetically identical copies of genes, cells, tissues or whole organisms. Cloning happens in nature and can also be done by people.

Natural clones

  • Bacteria and some plants reproduce asexually from a copy of one parent cell or part of a parent.
  • In humans and other mammals, identical twins arise when one fertilized egg splits into two embryos with nearly identical DNA. Twins are natural clones of each other (but not of either parent).

Cloning plants: vegetative propagation

Many plants clone themselves by vegetative propagation, producing new plants from a part of the parent plant. The offspring are genetically identical to the parent, and they skip flowers, pollination and seeds.

MethodHow it worksExample
Runners (stolons)Stems grow along the ground and form roots and buds where they touch soilStrawberry
TubersSwollen underground stems; each “eye” can sprout a new plantPotato
Bulbs and cormsUnderground storage structures that give rise to new plantsTulip, daffodil, garlic
RhizomesUnderground stems that produce several plantsGinger, iris
CuttingsA stem section with nodes is rooted in moist soil or waterMany garden plants
LayeringA bent stem is buried (or wrapped) until it roots, then separatedMany shrubs
Tissue cultureSterile plant cells grow on nutrient medium into many plantletsRare, endangered or disease-free plants

Cloning animals

Scientists distinguish three kinds of artificial cloning. Gene cloning copies a single gene or DNA segment. Therapeutic cloning makes cloned embryos only to collect stem cells. Reproductive cloning creates a whole animal, by these steps:

  1. A mature body cell, such as a skin cell, is taken from the animal to be copied.
  2. The nucleus of that cell is moved into an egg cell whose own nucleus has been removed.
  3. The egg develops into an early embryo in a laboratory dish.
  4. The embryo is placed in a female animal, a surrogate mother, who gives birth to an offspring genetically matching the donor.
How an animal can be cloned1. Body cellfrom the animalto be copied2. Nucleus put intoan egg that hasno nucleus3. Egg growsinto an earlyembryo in a dish4. Embryo placedin a surrogatemotherDNA fromthe donordonor DNAin the eggcells divideby mitosisoffspring matchesthe donor's DNAThe young animal is a genetic match of the donor, not of the egg donor or surrogate.
Reproductive cloning of an animal by moving a body-cell nucleus into an egg (simplified).

The best-known example is Dolly the sheep, produced in 1996 by Scottish researchers from a cell of the udder of a 6-year-old sheep. She was the first mammal cloned from an adult body cell. Only one of 277 cloned embryos was born alive, and Dolly died at six years old, about half the typical lifespan of a sheep. Success rates of cloning are low, and surviving clones have shown health problems.

Common misconception

“A clone is a perfect, instant copy of an adult.” A clone shares the donor's DNA, but it starts life as an embryo and grows up in a different time and environment, so it will not be an exact replica of the donor's personality or experiences. Human cloning claims have also not been supported by solid evidence.

6Grafting: joining two plants into one

Grafting is an artificial method of propagating plants in which a piece of one plant is joined onto another plant so that the two grow as one. Two parts are involved.

  • The scion is a stem piece (a twig or bud) cut from a plant with desirable traits, such as tasty fruit.
  • The stock (rootstock) is the rooted plant onto which the scion is attached.

When the cut surfaces are held together, the transport tissues (the vascular tissue) of the two pieces fuse. Water and nutrients from the stock's roots then travel into the scion, and the scion produces the desired fruit or flowers. Grafting is common in grape growing and the citrus industry.

Grafting a scion onto a stock1. Stock and scion(separate pieces)2. Scion fitted into acut in the stock3. Tissues fuse; the sciongrows on the stock's rootsThe scion keeps its own traits, so its fruit or flowers stay the same.
Grafting: a scion with desired traits is joined to a rooted stock.
Worked example

A gardener has a fruit tree that produces excellent fruit. Planting its seeds is unlikely to give trees with the same fruit, because seeds come from sexual reproduction and each seedling is a new genetic mixture. Instead, a twig is cut from the favourite tree (the scion) and grafted onto the rooted trunk of another tree (the stock). The new branch is genetically identical to the favourite tree, so its fruit has the same characteristics, and it benefits from the stock's established roots.

Grafting is classified as asexual reproduction because the scion is simply a part of one parent plant, continuing to grow with the same genes. No fertilization occurs.

Other asexual methods in the animal world

The curriculum names five forms, but biologists describe others too. Two are worth knowing.

  • Fragmentation. The body breaks into pieces, and each piece that is large enough regenerates into a complete individual. Sea stars, sponges and some worms do this. A fisheries effort to control sea stars by chopping them in half backfired because both halves regenerated.
  • Parthenogenesis. An unfertilized egg develops into an individual. It is used by water fleas, aphids, stick insects and some ants, wasps and bees. In bees, unfertilized eggs become male drones. Isolated female Komodo dragons and some sharks have produced young this way.

7Strengths and weaknesses of asexual reproduction

AdvantagesDisadvantages
Only one parent is needed, so no mate has to be foundOffspring are genetically identical, so there is little variation
Rapid reproduction when conditions are goodA disease or change that harms one individual can harm all of them
Offspring are already suited to the habitat that suited the parentNew variation comes only from rare mutations
Can colonize new habitats from a single individualMulticellular organisms that rely only on asexual reproduction are very rare

Asexual reproduction works best in a stable environment, because offspring identical to a successful parent are likely to succeed as well. In an unpredictable environment, the lack of variation is a risk.

Key idea

The five forms of asexual reproduction all produce clones, but they work differently. Fission splits the parent into two similar halves. Budding grows a small copy on the parent. Spores are single cells that can each grow into a new organism. Cloning is the general term for producing genetically identical copies, whether naturally or by people. Grafting joins a piece of one plant onto the roots of another so that it grows as one plant.

Comparing the five forms

FormWhat happensExampleOffspring identical to parent?
FissionParent splits into two similar individualsBacteria; sea anemonesYes (apart from rare mutations)
BuddingOutgrowth develops into a new individualYeast, hydraYes
SporesSingle reproductive cells made without fertilization grow into new individualsAsexual spores of mouldsYes (asexual spores)
CloningGenetically identical copies of organisms or cells are madeStrawberry runners; cloned sheepYes (same DNA)
GraftingScion joined to a rooted stockGrapes, citrus treesScion is identical to its source plant

Investigating asexual reproduction

Asexual reproduction offers many chances for simple investigations. Questions that could be tested include: Does the amount of sugar change how fast a yeast culture buds? Do cuttings from different parts of a stem root equally well? Does a potato piece with an “eye” sprout while a piece without one does not?

A fair test changes one factor (the independent variable), measures one outcome (the dependent variable, such as the number of buds counted under a microscope or the length of roots after ten days) and keeps everything else the same. Repeating the test with several samples helps to show whether a difference is real or just chance. These skills fit the curricular competencies of questioning, planning an investigation, and evaluating its reliability.

The next two lessons look at the alternative: sexual reproduction, which needs a different type of cell division (meiosis) and produces offspring that differ from both parents.

🔑Key terms

asexual reproductionReproduction by one parent that produces genetically identical offspring.
cloneAn individual or cell that is genetically identical to its parent or source.
binary fissionDivision of a bacterial cell into two cells after its DNA is copied.
septumThe dividing wall that forms across a bacterial cell during fission.
generation timeThe time a population takes to double by one round of cell division.
buddingFormation of an outgrowth on a parent that develops into a new individual.
sporeA tiny reproductive cell that can grow into a new individual without fertilization.
vegetative propagationGrowing new plants from parts of a parent plant.
reproductive cloningCreating a whole animal by moving a body-cell nucleus into an egg with its nucleus removed.
scionThe stem piece with desired traits that is joined to another plant in grafting.
stock (rootstock)The rooted plant that receives the scion in grafting.
fragmentationBreaking of the body into pieces that each regrow into a complete individual.

?Quick check

Try each question first, then reveal the answer.

1. Why are the offspring of asexual reproduction genetically identical to the parent?

2. Describe the four main steps of binary fission.

3. A bacterial culture starts with 2000 cells and doubles every 20 minutes. How many cells are present after 1 hour, assuming no deaths?

4. How does budding differ from fission?

5. Explain how an asexual spore differs from a sexual spore.

6. Name the parts joined in grafting and explain why a grower might graft instead of planting seeds.

7. Give two examples of natural clones.

8. Evaluate: a farmer plants a field with clones of a single potato variety. Give one advantage and one risk.

BC curriculum content covered in this lesson
  • Different forms of asexual reproduction: fission, budding, cloning, spores, grafting
  • Sample inquiry question: What are the advantages and disadvantages of sexual and asexual reproduction? (asexual side)

References

  1. BC Ministry of Education and Child Care. Science 9 curriculum (Big Ideas, Content, Curricular Competencies). Accessed October 7, 2026.
  2. OpenStax. Biology 2e, 10.1 Cell Division. Accessed October 7, 2026.
  3. OpenStax. Biology 2e, 43.1 How Animals Reproduce. Accessed October 7, 2026.
  4. OpenStax. Microbiology, 9.1 How Microbes Grow. Accessed October 7, 2026.
  5. Lumen Learning (Biology for Majors II). Asexual Reproduction. Accessed October 7, 2026.
  6. Lumen Learning (Biology for Majors II). Asexual Reproduction in Plants. Accessed October 7, 2026.
  7. Encyclopaedia Britannica. Reproductive processes of fungi. Accessed October 7, 2026.
  8. National Human Genome Research Institute. Cloning Fact Sheet. Accessed October 7, 2026.

These lessons follow the content areas listed in the British Columbia curriculum. They are study material written for this site and are not an official document. The official curriculum is the authority on what each course requires. Lessons are general education, not medical advice.